PCR Food Testing
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PCR food testing is the engagement of
Polymerase Chain Reaction The polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies (complete or partial) of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it (or a part of it) t ...
(PCR) technologies for the testing of
food Food is any substance consumed by an organism for nutritional support. Food is usually of plant, animal, or fungal origin, and contains essential nutrients, such as carbohydrates, fats, proteins, vitamins, or minerals. The substance is inge ...
for the presence or absence of
human pathogen A human pathogen is a pathogen (microbe or microorganism such as a virus, bacterium, prion, or fungus) that causes disease in humans. The human physiological defense against common pathogens (such as ''Pneumocystis'') is mainly the responsibility ...
s, such as
E. coli ''Escherichia coli'' (),Wells, J. C. (2000) Longman Pronunciation Dictionary. Harlow ngland Pearson Education Ltd. also known as ''E. coli'' (), is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus ''Escher ...
,
Salmonella ''Salmonella'' is a genus of rod-shaped (bacillus) Gram-negative bacteria of the family Enterobacteriaceae. The two species of ''Salmonella'' are ''Salmonella enterica'' and ''Salmonella bongori''. ''S. enterica'' is the type species and is fur ...
,
Listeria ''Listeria'' is a genus of bacteria that acts as an intracellular parasite in mammals. Until 1992, 17 species were known, each containing two subspecies. By 2020, 21 species had been identified. The genus is named in honour of the British pi ...
, etc. Four sample collection sites for PCR food testing can be: # The food irrigation water. # The food wash water. # Environmental samples collected in the food processing facility. # The finished food product, whether fresh or processed. Each of these sample types can be collected, prepared and PCR tested within a short time for many sample types. Some sample types may require sample enrichment via shortened culture growth periods prior to PCR testing.


References


External links

*https://www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalManualBAM/UCM070080 *https://www.fda.gov/Food/GuidanceComplianceRegulatoryInformation/GuidanceDocuments/ProduceandPlanProducts/ucm120246.htm#ftn1 *http://ftp.sccwrp.org/pub/download/DOCUMENTS/JournalArticles/484_pcr_fecal_santamonica.pdf {{DEFAULTSORT:PCR food testing Product testing Industrial processes Food industry Food science
Processing Processing is a free graphical library and integrated development environment (IDE) built for the electronic arts, new media art, and visual design communities with the purpose of teaching non-programmers the fundamentals of computer programming ...
Agricultural economics